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AOCHUANG CHEMICAL

Glucose oxidase

Glucose oxidase is an enzyme found in molds and honey such as Penicillium notatum. It catalyzes the reaction of D-glucose + O2D-gluconic acid (δ-lactone) + H2O2. EC1.1.3.4. The enzyme of P. notatum has attracted attention due to its apparent antibacterial properties. Therefore, there is also a name for glucose oxidase (notatin), and it is now clear that the antibacterial property is due to the sterilization effect of H2O2 produced by the reaction. The purified product contains 2 molecules of FAD and acts as an electron acceptor. In addition to O2, it can also react with 2,6, dichlorophenol and indophenol. This enzyme is specific for glucose. The Michaelis constant Km is also low (about 10-3 M), and since it can quantitatively generate H2O2, it is widely used as a quantitative reagent for D-glucose in the field of biochemistry and clinical examination.

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